Direct measurement of inositol in bovine myelin basic protein
نویسندگان
چکیده
منابع مشابه
Direct measurement of inositol in bovine myelin basic protein.
Myelin basic protein has been isolated from bovine central-nervous-system myelin by four methods, none of which exposes the protein to acid. After purification the inositol content of both hydrolysed and unhydrolysed protein was quantified by g.c.-m.s. Basic protein prepared by all methods contained less than 4 mol % of inositol. It is concluded, contrary to a previous proposal, that covalent b...
متن کاملSerum and Saliva Myelin Basic Protein as Multiple Sclerosis Biomarker
Objective: Multiple sclerosis (MS) is presented with motor and sensory function loss. It is caused by demyelination and following axonal lesion. As myelin basic protein (MBP) is one of the key elements of the myelin cover, we examined the level of MBP in serum, stimulated, and unstimulated saliva as a suitable biomarker for detecting MS. Methods: A case-control study was performed in 29 health...
متن کاملMyelin basic protein.
The fact that central nerve tissue is encephalitogenic has been known since the observation of a post-injection reaction of some rabies victims to the Pasteur vaccine that contained nerve tissue (Brostoff, 1977). Subsequently the encephalitogenic agent in central nerve tissue was traced to a component of the myelin sheath, and a protein was finally isolated that in the presence of complete Freu...
متن کاملThe sequential limited degradation of bovine myelin basic protein by bovine brain cathepsin D.
Bovine myelin basic protein (BP) microheterogeneous components were isolated and exposed to homogeneous bovine brain cathepsin D purified by affinity chromatography on immobilized pepstatin. The extent of the reaction was followed by polyacrylamide gel electrophoresis at pH 8.8, and the reaction products were separated by column chromatography on carboxymethyl-cellulose and Sephadex following w...
متن کاملPhosphorylation of myelin basic protein.
Myelin membranes prepared from rat brain possess both the enzyme and substrates to incorporate azP from (ya2P]ATP into membrane protein constituents. Qf the myelin polypeptides, only the two basic proteins were phosphorylated ; and both components appeared to be equally good substrates for endogenous or added protein kinases. The a2P was transferred primarily to serine residues of the basic pro...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1987
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bj2480285